ICSI Treatment in Hyderabad
Until the 90’s, males with very low sperm count (less
than 5 million per ml) or with poor quality sperms had no hope of fathering
their own genetic children. This problem was circumvented by a new
breakthrough, I.C.S.I. (Intra-cytoplasm Sperm Injection) an ART Treatment which
took place in Brussels and Belgium in 1992.
Since then, many such patients with poor sperm count or
with low quality sperm have been able to father their own genetic child through
ICSI treatment in Hyderabad. At Kiran
infertility centre, we started our own ICSI programmed in 1998-99 and until
date, more than 10,000 cycles of IVF or IUI with ICSI procedure in Hyderabad have been undertaken with an
approximate success rate of around 60% to 80% in each cycle, which is at par
with the best success percentages in this treatment across the globe.
In ICSI, all the steps are similar to that in the IVF procedure,
except the step of fertilization. Normally in IVF, extracted sperms, which are around,
or more than 200,000 and allowed to fuse with one egg and among these, one of
the sperm fertilizes the egg on its own. In contrast to this, while undertaking
ICSI, each egg is held and injected with a single live sperm. This
micro-fertilization is done with the help of a machine called the
Micromanipulator. The procedure can be categorized into 11 steps as discussed
below.
Thus, the procedure consists of:
ICSI treatment in India
·
Controlled ovarian
stimulation with drugs like (GnRH) Analogues and Gonadotrophins to produce multiple
follicles/ eggs
·
Monitoring of follicles and
egg development with the aids like Vaginal Sonography and serial Estradiol
hormone estimation through blood samples.
·
Administering of HCG
injection, (Human Chorionic Gonadotrophins) when at-least two leading
follicles, which are 18 mm in diameter
·
Egg retrieval under general
anaesthesia, approximately after 36
hours after HCG injection
·
Identification and isolation
of healthy eggs in the laboratory
·
Sperm collection and
processing in the lab (In case of Azoospermia {no sperms in the semen} the
sperms are collected directly from the testes with the procedures of
PESA/MESA/TESE or TESA)
·
Stripping of the cumulus of
the eggs in the laboratory with the help of an enzyme Placement of eggs into
small droplets of culture media under oil
·
Placing sperms into small
droplets of PVP under oil and then Immobilization of the sperm with a
microinjection needle (Diameter of 7 microns) and aspiration of the immobile
sperm into the needle after crushing the tail portion of the sperm)
·
Holding the egg with a
holding pipette and injecting of immobilized sperm into the held egg
·
Placement of these eggs into
the incubator for 2 to 5 days
·
Embryo formation 2 to 5 days
after fertilization
·
Embryo transfer of good
quality embryos back to the womb, on day-2 (four-cell embryo), day-3 (6 to 8
cell embryo) or day-5 or 6 (Blastocyst stage) days after egg removal.
Indications
for ICSI
·
Males with severe sperm
factors such as low sperm count (less than 5 million), very poor motility or a
high degree of abnormal sperms require Intra
Cytoplasmic Sperm Injection treatment in Hyderabad.
·
In Azoospermia Condition,
where there is no sperm present in the semen. Azoospermia may be of the
obstructive type where there is a production of sperms in the testes but
blockage of the transport system, which brings the sperm out into the semen.
Alternately, in Azoospermia condition may be of the non-obstructive type, where
there is a failure of the testes to produce sperms. In current scenario, in
both these conditions, sperms can be isolated directly from the testis by
Testicular Biopsy.
·
Retrieval Techniques of
PESA/TESA/TESE and subsequently, ICSI can be performed on the following cases:
,
o Males
with Sperm Ejaculated Dysfunction due to Spinal injury or malfunction such as
Quadriplegics or Paraplegics
o Males
with Anti sperm-antibodies
o Patients
with Retrograde Ejaculation (ejaculation of the sperm into the urinary bladder)
who fail to become pregnant with IUI.
o Patients where fertilization has failed with In-Vitro Fertilizatio
Intra cytoplasmic sperm injection has revolusionised the way Fertility treatments were conducted in the past. ICSI treatment in Hyderabad is world class and offered at the Kiran infertility centre in Hyderabad/bangalore and Chennai wherein even the most difficult of male infertility cases can be treated succesfully
